SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Supplier: Molecular Devices. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. 76358-624. Bookings are free, but you will need to use iLab to book. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. 020 fmol 0. Microplate Readers Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. Figure 2-23: Bottle holder To install the bottle holder: 1. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Additionally, user-installable options allow the SpectraMax i3 System to. SpectraMax® Microplate Readers and SoftMax® Pro Software. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. 2. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. SpectraMax® 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. microplates or in a cuvette at a. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Lysozyme contains two tryptophan residues that. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. We would like to show you a description here but the site won’t allow us. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. SpectraMax Microplate Readers. Path Check Pathlength Measurement every Gemini EM Reader. SpectraMax i3x Multi-Mode Detection Platform. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. For a higher . Download PDF versions of the SpectraMax Microplate Reader User Guides. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. SpectraMax Spectrofluorometers. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Prerequisites: SoftMax Pro 7. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. The University of Rouen in Normandy uses the SpectraMax iD3 and. Microplate Readers. When the user applies a set temperature of 37°C, the top is heated to 37. It also integrates with the. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. SpectraMax 250 User. The Gemini XS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. . The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. View the Molecular Devices website. The Gemini EM can sample a variety of formats, from 6- to 384-well. Microplate Readers. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Lower limits of detection for ATP determined using the ATPliteAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. 58% for the detection module and 2. Multimode Microplate Reader. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. ATP standard curves read on SpectraMax microplate readers. Add to Cart. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Figure 1. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Microplate Readers BioTek Epoch 2 Microplate Reader. . The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking andThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expandthe boundaries of your research capabilities. Microplate Automation & Detection. In . With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Our absorbance plate readers feature our PathCheck Sensor technology and. western blot membrane detection in a multimode microplate reader platform. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Compact and tunable absorbance microplate readers DATA SHEET. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Type:Spectrophotometer. Detection modalities include absorbance (UV-Vis Abs) and. The SpectraMax microplate readers can be used to accurately quantify ROS levels using a luminescence-based assay. Find regional support. Products : General, Microplate Readers. Maximum signal with lowest background. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Brochure. The calculated LOQ values were 0. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate 312 gain calibration failed. Add to Cart. Detailed specifications of each model reader are reported in Section 7, “Specifications”. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Nucleic Acid and Protein Quantitation. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 5 12. Introduction. MPN:SpectraMax Plus. 316 grating motor driver faulty. ” Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. The SpectraMax® ABS Plus Microplate Reader can run . Narrow 2nm bandwidths deliver increased measurement accuracy and linearity over the widest range. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraDrop microplate was read on the SpectraMax ® Paradigm ® Multi-Mode Microplate Reader using the Fluorescence Intensity detection cartridge (FI-FLRH) with fluorescein filters, or Tunable Wavelength Selection (TUNE) cartridge with signal/ background-optimized wavelengths. Examples: “Press carefully to engage,” “ Do not. Figure 1. Blank subtraction and standard curve fitting were done automatically by the software. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Fluorescence Microplate Reader Comparison Chart. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. begin reading microplates with seven mouse clicks. 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. . From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. For readers without a cuvette port, these options are not shown in the software. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 20 μL of Component A was added to each well of the microplate. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Cell Analysis. flexibility of monochromator-based systems. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Introduction. Read More. Open the top cover of the instrument. Dual-Glo luciferase assay system (Promega Cat. Table 1. both cuvette-based and microplate reader applications on the same instrument. 2. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual monochromators. Transcreener ® ADP. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Promega's ROS-Glo H202 assay can be combined with SpectraMax readers to explore the relationship between. 33 Dramatic aggregation of Alzheimer A b by Cu(II) is induced by conditions representing physiological acidosis . A: Microplate Preparation. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. In this product video, see how the SpectraMax M5 quickly reads samples. flexibility of monochromator-based systems. 303 unable to cal dark current. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. 7°C and the bottom to 36. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. Note that this procedure is only for the SpectraMax L model. 0 CART STOREVersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. The Quant-iT PicoGreen dsDNA Assay Kit, when run on a SpectraMax microplate reader with SoftMax Pro Software, is a quick, sensitive detection method for double-stranded DNA. When the user applies a set temperature of 37°C, the top is heated to 37. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. Large, high resolution touchscreen interface allows you to easily set up methods, run experiments or view tutorial videos without the need for a dedicated computer workstation. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. 313 reference gain check fail. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. microplate reader. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. Endpoint, kinetic, spectrum. SoftMax® Pro 7. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Italic and boldface type are used for emphasis. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Multimode Microplate Readers. 314 low lamp level warning. It also offers a field-upgradeable cellular imaging option and a cooled PMT for low-light sensitivity. This variable pathlength makes it dif Þcult to perform extinction-based assays and confusing to compare results between microplate readers and spectrophotometers. The SpectraMax 190 reader can be integrated with Molecular . 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. Add to Cart. It features a modular design that allows users to add or change detection cartridges according to their needs. SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. Win7 Manual Spectramax-L or StakMax Driver Installation after Initial Install. Sensitive fluorescent quantitation of DNA with the Quant-iT. Ease of use. Introduction. Compare plate readersFive-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of. SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. ELISA Plate Readers / Microplate Readers. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Figure 1. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Assays vary depending on. These lab workhorses are used in drug discovery. 306 plate air cal. Wide range of applications The VersaMax reader covers. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Align the two slots on the bottle holder with the two pegs on the rail. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The dual monochromator optics allow. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Please refer to the Obsolete Microplate Reader Models for more information. The system uses two scanning monochromators to determine the optimal excitation and emission settings. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. . Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. View your data quickly using the large touchscreen interface, export your data for analysis in the program of. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. This pathlength correction is accomplished only with the use of. 1. 1 software Download page. An assay plate containing a 15-point standard curve was read on the SpectraMax. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. SAN JOSE, CA — Molecular Devices, LLC. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and. For more sample throughput, the system can SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Examples: “Press carefully to engage,” “ Do not. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. M2. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. Results are shown in Table 1. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. The price range of a reader starts at 2. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. #6590-0131) Fluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The plate reader can measure multiple readouts, including UV and visible absorbance. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. Most papers they cultured bacteria to OD600>0. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for. 230-1000 nm, Monochromator, ABS, FLR (top and bottom) and LUM, with incubation. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Step 1: Turn on your microplate reader. A. SpectraDrop High-Throughput Screening (HTS) Kit. The chamber contains springs that position the cuvette in the proper alignment for a read. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. In addition, SoftMax Pro Softwareluminescence, and fluorescence microplate readers, and the SpectraMax® Paradigm® Multi-Mode Detection Platform. On the back, in the right hand top corner,. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Endpoint, kinetic, spectrum. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. The guide also covers the software features, the specifications, and the accessories of the systems. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. com. The powerful analysis tools in the software generate a standard curve and calculated data. The SpectraMax® iD3 and iD5 measure absorbance, fluorescence, and luminescence. 304 signal level saturation. Guides: SpectraMax Mini Installation Guide. No accessories are needed and SoftMax® Pro Software. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Avoid losing high signal. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. The Molecular Devices Gemini XS Fluorescent Microplate Reader lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for new assays. Wide range of applications. limit of detection (LLD) for ATP was estimated for each microplate reader using the . Figure 6: Transcreener ADP2 FI assay principle. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Highly customizable low volume. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Thank you!. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Absorbance (optical density, OD) was read at 570 nm and 605. Turn the reader off by pulling the power cord. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. 0 out of 5 $ 11,999. Fill a clean plate with water. 100 fmol 1536-well (8 µL) 0. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. Products. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The plate reader can measure multiple readouts, including UV. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. no. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. . An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. Other technologies such as imaging. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. 5 12. 58% for the detection module and 2. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . 1. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. Microplate reader assays requiring infrared (IR) or red-shifted. SpectraMax Mini User Guide. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. The screw remains inside the instrument. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. Add to Cart. The Spectramax 190 has an array of data collection modes, including kinetic, endpoint, and spectral scan modes. In addition, the SpectraMax iD5 can be expanded to. The Spectramax 250s xenon flash lamp and grating monochromator facilitates direct UV measurement of DNA, RNA, and protein in microplates. Microplate automation and detection instruments for walk away operation and high quality results. 2. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. This system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. Sale. The SpectraMax ABS Plus Microplate Reader is a compact and adaptive microplate reader capable of quantitating nucleic acid and protein samples in various formats such as cuvettes or microplates. on the SpectraMax iD3 microplate reader Introduction The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. Contents of wells were pipetted up and down several times to mix. Table 2 summarizes the results for the uniformity test. The SpectraMax L Luminescence Microplate Reader with the variable-volume injectors option (10 µL to 100 µL) can measure both flash and glow luminescence. Add to Cart. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. , April 14, 2022 – Molecular Devices, LLC. 0 out of 5 $ 23,995 $ 22,495. SpectraMax L Microplate Reader This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. This is equivalent to having 511 built-in filters,The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. FI assay. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast. The SpectraMax® ABS Plus Microplate Reader can run . 4 μL DNA standards. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. 72 MB. The CellTiter-Glo assay from Promega uses luciferase.